Fluorescence resonance energy transfer (FRET) sensors
نویسندگان
چکیده
منابع مشابه
Fluorescence resonance energy transfer (FRET) sensors
The principle of "fluorescence resonance energy transfer" (FRET) has been exploited in cell biology to demonstrate the interaction of two proteins in living cells. FRET is highly specific and sensitive and allows for the online recording of events that modulate the interaction. We here demonstrate that FRET-based sensor molecules represent an alternative in monitoring receptor-dependent signall...
متن کاملFluorescence Resonance Energy Transfer (FRET) sensor
The FRET has been widely used as a spectroscopic technique in all applications of fluorescence including medical diagnostics, DNA analysis, optical imaging [1] and for various sensing properties [2-11]. Generally, fluorescence-based sensors adopt three different strategies: (a) fluorescence quenching (turn-off), (b) fluorescence enhancement (turn-on) and (c) ratiometric FRET. FRET sensors becam...
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Each of the two domains of mammalian metallothioneins contains a zinc-thiolate cluster. Employing site-directed mutagenesis and chemical modification, fluorescent probes were introduced into human metallothionein (isoform 2) with minimal perturbations of the structures of these clusters. The resulting FRET (fluorescence resonance energy transfer) sensors are specific for each domain. The design...
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Fluorescence resonance energy transfer (FRET) is an extremely effective tool to detect molecular interaction at suboptical resolutions. One of the techniques for measuring FRET is acceptor photobleaching: the increase in donor fluorescence after complete acceptor photobleaching is a measure of the FRET efficiency. However, in wide-field microscopy, complete acceptor photobleaching is difficult ...
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The complex organization of the transcription machinery has been revealed mainly by biochemical and crystallographic studies. X-ray structures describe RNA polymerases and transcription complexes on an atomic level, but fail to portray their dynamic nature. The use of fluorescence techniques has made it possible to add a new layer of information to our understanding of transcription by providin...
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ژورنال
عنوان ژورنال: BMC Pharmacology
سال: 2008
ISSN: 1471-2210
DOI: 10.1186/1471-2210-8-s1-a44